Part:BBa_K1333110

recA730 CDS

RecA730 is a part of the SOS pathway.It is a mutant of RecA which is also a active form of RecA.

Usage and Biology

When DNA encounters severe damage which may leads to critical problem of the cell, the most prior task is to stabilize the DNA as soon as possible, even at the cost of generate mutation. SOS bypass is an emergency DNA repair method of cells. The functional enzyme of SOS is polymerase V. When the external signal ,like UV, activate the RecA coprotease activity, it can help the lexA gene to cleave itself, which will result in several genes to be induced. UmuD and UmuC are two of the induced genes. The product of UmuD gene will be cleaved by a proteinase and turn into UmuD’. The complex of UmuD’ and UmuC has DNA polymerase activity, which is referred as polymerase V.

SOS error prone bypass

For more information please visit our wiki

http://2014.igem.org/Team:SYSU-China/content.html#Project/Result/Mutagenesis

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 127
  Illegal AgeI site found at 631
  
• 1000
  COMPATIBLE WITH RFC[1000]